Autophagy and innate immunity.

munity-related GTPase M) and NOD2 (nucleotide-binding oligomerization domain 2)] results in the development of immune-related diseases, such as Crohn’s disease, a chronic inflammatory bowel disease [2] . Autophagy represents an ancient immune defense strategy present in flies. Importantly, as flies lack an adaptive immune system, the function of autophagy in cellintrinsic innate immunity can be studied in isolation without the added complexity of adaptive immunity. In addition to the autophagic pathway, innate immune pathways are conserved between flies and mammals. The use of flies to interrogate immune defense pathways has significantly enhanced our understanding of antimicrobial autophagy due in large part to the sophisticated genetic tools available in flies [3] . Beyond this conservation, it should be emphasized that signaling pathways that control innate immunity also regulate autophagy. In recent years, the autophagy membrane trafficking itself has been analyzed in terms of targeting, docking and fusion membrane events. Deciphering the signaling associated with membrane trafficking of infectious microorganisms in relation to autophagy during infection will help to better understand the tuning of the immune response [4] . The notion of autophagy as a purely degradative pathway was recently challenged by the emergence of studies that have Different and timely aspects of the role of autophagy and autophagy proteins in innate immunology are reviewed by leading authors in the field in this issue of the Journal of Innate Immunity. Indeed, autophagy has recently turned out to play a critical role in host defense responses by promoting the elimination of pathogens and the induction of acquired immunity. Macroautophagy (hereafter referred to as autophagy) is a form of autophagy that starts with the biogenesis of a double membrane-bound autophagosome that sequesters a fraction of the cytoplasm in a bulk or in a selective manner to deliver cargo to the lysosome [1] . Because autophagy is the only known mechanism to remove cytoplasmic contents that are larger than can be captured by the proteasome, it has also been proposed as a likely component of the cell’s arsenal against infectious organisms. Indeed, protein degradation is critically involved in the control of innate immune responses. Autophagosome formation is under the control of Atg (autophagy-related) genes that are conserved during eukaryotic evolution. Recent advances in the study of Atgs provide strong evidence that they influence both the host defense response and the regulation of inflammation. Consistently, it has been proposed that the compromised function of autophagy regulators [ATG16L1, IRGM (imPublished online: June 15, 2013 Journal of Innate Immunity